Abstract
Hybridization patterns of specific cloned DNA sequences to restricted genomic DNA are analyzed in DNA fingerprinting, a technique used, e.g., for identification of individuals and for studying inheritance. We have studied the genetic variability in basket willow (Salix viminalis) by DNA fingerprinting using a bacteriophage M13 DNA probe. The five independent genotypes (clones) studied could be distinguished from each other in different enzyme digests. In three of the genotypes, restriction fragments were found that were not present in any of the other clones. The hybridization patterns to the M13 probe were somatically stable within the clones. The patterns were identical among different individuals within each clone, and remained unchanged in callus cultured in vitro, including genetically transformed callus lines. Three progeny (one individual of each) derived from two different crosses were analyzed. All the fragments seen in the offspring were present in one or both of the parents, and there are indications of homo- and heterozygosity in loci corresponding to some of the fragments. The results show that the method is highly sensitive and has a potential to be used in genotype identification in willow.
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