Abstract
Introduction: Ber (Ziziphus nummularia (Burm. F.) is the most commonly occurring branched thorny shrub species in the Indian desert. A total of 10 Zadber accessions collected from different agroclimatic regions of India, were evaluated for phenotypic and genotypic variations using morphological and inter-simple sequence repeats (ISSR) markers. Materials And Methods: Morphological characterization was done using descriptors and Molecular characterization studies was done with fifty primers of University of British Columbia (UBC) procured from Geno Biosciences Pvt. Ltd., were used for ISSR-PCR optimisation trials. Eleven primers, which gave the best amplification results with the sample DNA, were selected for final ISSR-PCR analysis. Results: Significant variability was observed in the selected Zadber accessions by the analysis of five quantitative and 25 qualitative morphological characters of leaves, fruits and seeds. ISSR markers also showed polymorphism (86.58%). Jaccard’s genetic similarity value of ISSR was found in the range of 0.45-0.77 (average 0.61) suggesting moderate level of genetic diversity within the Zadber group. Two of the eleven ISSR primers were also able to generate cultivar specific amplicons, which may be used for identification of accessions Zadber-5 and Zadber-8. Conclusion: The present study revealed that morphological and molecular markers can be successfully utilized for determining genetic diversity and genetic relationship of Zadber cultivars and used in breeding programmes.
Highlights
IntroductionOf the well-known species of the genus Ziziphus, ber (Z. nummularia (Burm.f.) is the most common in the tropical and sub-tropical regions
We report here the genetic diversity assessed using random amplified polymorphic DNA (RAPD) and ISSR markers with the comparison of the two marker systems to evaluate the genetic relationship among the 10 accessions belonging to Z. nummularia on the basis of leaf sample
Based on ISSR markers, high similarity value of Findings of the present study revealed that 10 zadber germplasm were moderate to high diversity based on molecular and morphological assessment approaches
Summary
Of the well-known species of the genus Ziziphus, ber (Z. nummularia (Burm.f.) is the most common in the tropical and sub-tropical regions. Among the various marker systems, the randomly amplified polymorphic DNA (RAPD) is one of the most popular DNA-based approaches.[11,12,13,14] The ability of Ziziphus species and different varieties of mauritiana has study to cross freely to build up of rich gene pool that depicts heterozygosity in their adaptability to soil and climate; morphological, physiological and phenological traits; chromosome number; tolerance/resistance to biotic and abiotic stresses and genomic DNA.[15] The RAPD technique is a potentially simple, rapid, reliable and effective. The ISSR markers are generated from single-primer PCR reactions and the primer is designed from
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