Genetic variability and molecular responses of root penetration in cotton

Abstract

Compacted soils restrict root penetration hindering productivity. In this paper, genetic variability of cotton ( Gossipium spp.) root capacity to penetrate hard soil layers and the patterns of gene expression during penetration event were investigated. To mimic hard soil layers, wax–petrolatum mixtures were used. Genetic variability among 27 cotton genotypes for the root capacity to penetrate wax–petrolatum disks of 500–700 g wax/kg of mixture was high indicating that breeding efforts targeted to improve this trait can be successful. In the root tips of a cotton strain with high root penetrating ability ( G. hirsutum HS 200) which penetrated through wax–petrolatum disks (P), quantity of four polypeptides with molecular weights 35–66 kDa increased compared to those root tips which grew in the absence of mechanical impedance (NP). Differential display showed significant differences in the sets of mRNA expressed in P and NP roots. Out of a total of 917 cDNAs scored in the differential display experiment, 118 cDNAs, or 13%, were specific to P roots and hence could be associated with the root penetration event. Further detailed study of gene expression in penetrated roots will pinpoint molecular factors involved in root penetration ability in cotton.