Genetic variability analysis of Chinese and Hungarian northern pike (Esox lucius L.) based on microsatellite markers

Abstract

Genetic variability of northern pike(Esox lucius L.) was investigated in three populations(Ulungur Lake,Jili Lake and No.6 Lake) in Xingjiang,China and one population(Balaton Lake) in Hungary by using 18 microsatellite markers.The results indicated that the mean allele richness(AR),mean observed heterozygosity(HO) and expected heterozygosity(HE) of the Chinese populations were significantly lower than those of the Hungarian population(P0.05),whereas the mean inbreeding coefficient(FIS) of the Hungarian population was higher than that of the Chinese populations.Significant bottleneck signal was detected from Hungarian population by using SMM and TPM model(P0.001).Analysis of molecular variance and pairwise FST values indicated that there was distinct genetic difference between Chinese and Hungarian populations(P0.01).The results of Neighbour-joining tree,Principal Coordinate Analysis(PCA) further confirmed that there was obvious genetic difference between Chinese and Hungarian populations.Furthermore,the results of Bayesian cluster analysis showed that northern pikes in the No.6 Lake would have been introduced from the Ulungur Lake rather than from the Jili Lake.