Abstract
ABSTRACT Broccoli (Brassica oleracea var. italica) is one of the most important vegetables in the world and also shows important functional properties. The present study aimed to characterize 19 broccoli genotypes using biochemical traits (content of total phenolic compounds, total flavonoids and antioxidant activity) and AFLP (Amplified Fragment Length Polymorphism) molecular markers. The experiment was carried out in a greenhouse using a complete randomized block design with three replicates. The phenotypic data were subjected to analysis of variance (p≤0.05), Scott-Knott average clustering method (p≤0.05) and principal component analysis (PCA). Using molecular data, Ward’s hierarchical clustering, Bayesian clustering and principal coordinate analysis (PCoA) were performed. Molecular data showed genetic diversity among the genotypes (three groups), also a wide variability in the total phenolic compounds, total flavonoids content and antioxidant activity using FRAP method. HT3010, Hanabi and Bonanza genotypes showed desirable biochemical traits for the demanding functional food consumers, in addition to being promising genotypes to be exploited in plant breeding programs.
Highlights
Brócolis (Brassica oleracea var. italica) é umas das principais hortaliças do mundo e apresenta importantes propriedades funcionais
Broccoli germplasm is divided into two main commercial groups: ramoso, which presents several small lateral inflorescences, and single-head broccoli, showing inflorescences concentrated at the shoot tip, known as Japanese broccoli (Ordiales et al, 2017)
We evaluated 19 genotypes of broccoli, being 12 single-head broccoli genotypes and 7 ramoso broccoli genotypes (Table 1)
Summary
Brócolis (Brassica oleracea var. italica) é umas das principais hortaliças do mundo e apresenta importantes propriedades funcionais. O presente estudo teve como objetivo caracterizar 19 genótipos de brócolis por meio de caracteres bioquímicos (teor de compostos fenólicos totais, flavonoides totais e atividade antioxidante) e marcadores moleculares do tipo AFLP (Amplified Fragment Length Polymorphism). Broccoli consumption has been increasing in Brazil in the latest years, due to its reasonable price, making it affordable for different social classes, and for its high nutritional value (Thomas et al, 2017). It is an important source of calcium, magnesium and essential amino acids (Melo, 2015; Chen et al, 2018). The aim of this study is to characterize broccoli genotypes using biochemical and molecular traits using AFLP markers
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