Abstract
Stable transformation of Eucalyptus globulus after biolistic DNA delivery was investigated using zygotic embryos as target material. Conditions for plant regeneration were first investigated. Six-day-old cultured embryos, which had shown to be a good target for DNA transient expression, appeared to be suitable for regeneration. Whole plants were recovered through organogenesis after particle gun bombardment. Transformation experiments were performed with a linear T-DNA fragment harbouring GUS and NPTII genes, using the biological and physical conditions defined for optimum transient expression. After 2 months on a culture medium, neoformed GUS-positive calli were obtained from the 'T-DNA' bombarded embryos. GUS-expressing calli were also recovered after selection with kanamycin following bombardment. The integration of both GUS and NPTII genes into the Eucalyptus genome was confirmed by Southern blot analysis, demonstrating stable transformation of Eucalyptus globulus cells for the first time.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
