Abstract
The present study included isolation of Sinorhizobium meliloti bacteria from the root nodules of Alfalfa plants, Transformation process was conducted by conjugation with transformed bacteria Agrobacterium tumefaciens. The results showed the efficiency of the process, where the antibiotic tests acquire two genetic labels, resistance to chloramphenicol and rifampicin on the conjugated strain, proved that Ti plasmid (Tumor-inducing Plasmid) transferred from the A. tumefaciens as well as that the conjugated strain produces melanin pigment. For more assurance, Alfalfa plants were inoculated with the conjugated strain, and the results showed the success of conjugation via crown gall formation on stem which formed by Ti plasmid in addition to root nodules in same plant. These results indicate the capability of performing conjugation technique between different species of Rhizobiaeceae to acquire new isolates carrying beneficial characteristics that are able to be transfer to plants and produce a new desired genetic transformed plants.
Highlights
The present study included isolation of Sinorhizobium meliloti bacteria from the root nodules of Alfalfa plants, Transformation process was conducted by conjugation with transformed bacteria Agrobacterium tumefaciens
تلقيح العائل النباتي بالبكتريا الناتجة من الاقتران أثبتت الاختبارات الحيوية من خلال تلقيح بادرات نبات ألجت التي بعمر )أسبوعين إلى ثلاثة أسابيع( بالسلالة المقترنة عن طريق غمر جذور البادرات بلقاح البكتريا وأيضا عن طريق إحداث جروح وحقنهـا فيه ،وبعد تحضين البادرات في ظروف مناسبة و مراقبة مستمرة ظهور استجابة لتكوين العقـد الجذريـة خلال 12-7يوم من فترة التحضين بينما تأخر ظهور الأورام إلى ما بعد الأسبوع الثالث من التحضين كما يظهر في الصورة رقم ) ،(1وهذا دليل واضح على حدوث عملية الاقتران ،كما أكد الكثير مـن البـاحثين نجاح عمليات نقل مبرمجة أو غير مبرمجة لجينات معينـة مـابين بكتريـا Agrobacteriumوبكتريـا
Summary
The present study included isolation of Sinorhizobium meliloti bacteria from the root nodules of Alfalfa plants, Transformation process was conducted by conjugation with transformed bacteria Agrobacterium tumefaciens.
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