Abstract
Efficient protocols for date palm embryogenic callus and somatic embryo transformation with uidA gene are described in this chapter. The embryogenic callus transformation procedure is 1.6μm gold particle size coated with 2.5μg DNA (pAct1-D plasmid), 1100psi helium pressure, 9cm target distance, 26inHg vacuum pressure, 3mm distance between the rupture disk and macrocarrier, and osmotic pretreatment with 0.4M mannitol followed by 60min air desiccation. The somatic embryo transformation procedure is 0.6μm gold particle size coated with 2.5μg DNA (pAct1-D plasmid), 1350psi helium pressure, 6cm target distance, 28 inHg vacuum pressure, 3mm distance between the rupture disk and macrocarrier, and osmotic pretreatment with 0.4M mannitol followed by 60min air desiccation. Protocols for analysis of the transgenic plantlets have also been described.
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