Genetic transformation and growth index determination of the Larix olgensis LoHDZ2 transcription factor gene in tobacco

Peiqi An,Lei Zhang,Ruofan Qin,Chen Wang,Qing Cao,Qingrong Zhao,Hanguo Zhang

doi:10.1038/s41598-021-99533-0

Peiqi An, Lei Zhang + Show 5 more

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https://doi.org/10.1038/s41598-021-99533-0

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Abstract

Homeodomain-leucine zippers (HD-Zip) are plant-specific transcription factors that participate in different plant development processes and differentially regulate metabolic processes. LoHDZ2 is an HD-ZipII subfamily transcription factor gene that we identified from a transcriptomic analysis of Larix olgensis. To understand its function, we built a LoHDZ2 expression vector and then inserted it into tobacco by genetic transformation. Transgenic plants were identified at the DNA and RNA levels. Phenotypic index analysis of transgenic tobacco showed dwarfed growth with larger leaves and earlier flowering than the wild type. LoHDZ2 was expressed differently after hormone treatment with IAA, MeJA and 2,4-D. The results suggested that LoHDZ2 may respond to hormones and be involved in regulating growth and metabolism. These results helped us better understand the function of LoHDZ2 and provided a candidate gene for Larix olgensis molecular breeding.

Highlights

Homeodomain-leucine zippers (HD-Zip) are plant-specific transcription factors that participate in different plant development processes and differentially regulate metabolic processes
After Larix olgensis seedlings were treated with 50 mg·L−1 GA3, IAA, ABA, 6-BA, MeJA, or 2,4-D solutions or water, according to the qRT-PCR results(Fig. 2, and see Supplementary information), it showed that the expression of LoHDZ2 was downregulated sixfold after treatment for 96 h with 6-BA
Based on the available data, no studies have reported on the function of HD-Zip family genes in Larix olgensis

Summary

Introduction

Homeodomain-leucine zippers (HD-Zip) are plant-specific transcription factors that participate in different plant development processes and differentially regulate metabolic processes. LoHDZ2 is an HD-ZipII subfamily transcription factor gene that we identified from a transcriptomic analysis of Larix olgensis. The results suggested that LoHDZ2 may respond to hormones and be involved in regulating growth and metabolism These results helped us better understand the function of LoHDZ2 and provided a candidate gene for Larix olgensis molecular breeding. Genetic structure and physiological function, HD-Zip transcription factors can be separated four subfamilies, such as HD-ZipI, HD-ZipII, HD-ZipIII and HD-ZipIV4. Different subfamilies have differences in their genetic composition and the structure of their encoded proteins These transcription factors participate in different plant development processes and differentially regulate metabolic processes. HD-ZipII transcription factors affect rice plant development through modulating gibberellin b iosynthesis[8]

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