Abstract
The transfer of genetic information into acidophilic (acid requiring) bacteria, and the production of suitable vectors for these cells are being investigated in order to develop genetic engineering systems for them. Such cells may prove useful in the biological desulphurization of coal, and these procedures are needed to take full advantage of their capabilities. Plasmids from different incompatibility groups were tested to determine the frequency with which they are transferred from Escherichia coli to Acidiphilium strains. IncP1 and IncQ plasmids were transferred at moderate efficiencies, whereas IncW plasmids were transferred much less efficiently. A conjugative function has been discovered in Acidiphilium strains by observing the transfer of mobilizable, but not self-transmissible, plasmids between Acidiphilium cells. Finally, a small, cryptic plasmid from Thiobacillus ferrooxidans has been cloned and partially characterized.
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