Abstract
Traceability based on DNA analysis is attracting increasing interest due to the crisis of confidence that consumers show towards the products of animal origin. The present work discusses a genetic traceability system of meat and processed products from an historical Tuscan native pig breed, the Cinta Senese. The study is based on a panel of 8 ISAG (International Society for Animal Genetics) DNA microsatellite markers usage done both on pigs and derived products. The SSRs panel allowed us to obtain a unique fingerprint of the individuals to be used as a tracer “downstream” in the processed products. The molecular method used proved that the hams, analyzed just before commercialization, were obtained from Cinta Senese pigs and that the analyzed meat products derived from the Cinta Senese were produced at least with 95% of Cinta Senese meat. In perspective, the molecular testing could be introduced as a voluntarily adopted method for proving intrinsic quality of many regional food products.
Highlights
The Cinta Senese—(CS) is an autochthonous pig (Sus scrofa domesticus L.) breedHow to cite this paper: Scali, M., Vignani, R., Quintetti, M., Bigliazzi, J., Paolucci, E. and Cresti, M. (2015) Genetic Traceability of Cinta Senese Pig (Sus scrofa domesticus L.): A Study of the Meat and Processed Products by Microsatellite Markers
The GenElute Mammalian Genomic DNA Kit was used to extract genomic DNA from each of the following samples always analyzed in triplicate: fresh CS ham belonging to a butchery batch that included, among others, the six individuals used for the meat analysis (Ham1 or Prosciutto1); pigskin coming from the same fresh CS ham (Pigskin1 or Cotenna1); aged CS ham not belonging to the butchery batch of the six individuals used for the meat analysis (Ham2 or Prosciutto2)
The present study focused to the development of a genetic traceability plan along the CS supply chain, from meat to the processed products
Summary
The Cinta (meaning belt) Senese (of Siena)—(CS) is an autochthonous pig (Sus scrofa domesticus L.) breedHow to cite this paper: Scali, M., Vignani, R., Quintetti, M., Bigliazzi, J., Paolucci, E. and Cresti, M. (2015) Genetic Traceability of Cinta Senese Pig (Sus scrofa domesticus L.): A Study of the Meat and Processed Products by Microsatellite Markers. The Cinta (meaning belt) Senese (of Siena)—(CS) is an autochthonous pig (Sus scrofa domesticus L.) breed. (2015) Genetic Traceability of Cinta Senese Pig (Sus scrofa domesticus L.): A Study of the Meat and Processed Products by Microsatellite Markers. Originating in Tuscany from around the areas of Siena, it is believed that this ancient hardy breed is more than likely bred in Roman times, the earliest acknowledgements found are dating from the Middle Ages. Up and into the 1950’s many people owned one or more CS for their meat, but a period of decline of the CS was attributed to a massive cross breeding with white pig breeds, which needed to be kept for a much shorter period than the CS before butchering. Large White (LW), that was introduced to Italy from the UK and Landrace (L) that was introduced mainly from several Scandinavian Countries
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