Abstract
Spinibarbus caldwelli was an endemic species to China, and its germplasm protection and resources utilization had become more and more concerned. To know its genetic diversity and differentiation, the mitochondrial DNA D-loop was amplified and sequenced for 148 individuals from four regions of Pearl River and Yangtze River Basin. Altogether 9 variable nucleotide sites existed among the aligned sequences of 748 bp, and 8 haplotypes were found within 148 individuals. The average nucleotide diversity (Pi) was high 0.00297, while haplotype diversity (Hd) was 0.706. The average genetic distance was 0.00298, most value occurred between LJ and CL populations, and small value occurred between HJ and QZ populations.
Highlights
Spinibarbus caldwelli is a teleost fish species belonging to the order Cyprinformes
Spinibarbus caldwelli was an endemic species to China, and its germplasm protection and resources utilization had become more and more concerned
9 variable nucleotide sites existed among the aligned sequences of 748 bp, and 8 haplotypes were found within 148 individuals
Summary
Spinibarbus caldwelli is a teleost fish species belonging to the order Cyprinformes. It is an important economic freshwater fish mainly distributing in the Yuanjiang, Yangtze, Jiulong, and Minjiang River, Pearl River basin and Hainan Island [1]. The gene diversity of S. caldwelli has been seriously depleted and the population has been seriously degraded. It has been listed in the Red Book of Endangered Species by the World Conservation Union [2]. There were many studies on the description, classification, age and growth, reproductive biology and diet of S. caldwelli [3] [4] [5] [6].
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