Genetic structure in natural populations of Dacrydium elatum (Roxb.) Wall. (Podocarpaceae) in the Central Highlands of Vietnam inferred by Microsatellites

Dinh Duy Vu,Mai Phuong Pham,Quoc Khanh Nguyen

doi:10.1051/e3sconf/202126501030

Dinh Duy Vu, Mai Phuong Pham + Show 1 more

Open Access

https://doi.org/10.1051/e3sconf/202126501030

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Abstract

To provide a reference for the conservation and application of breeding parentallines resource of Dacrydium elatum (Roxb.). Genetic diversity and population structure of eighty individuals from four populations (Kon Tum, Gia Lai, Dak Lak and Lam Dong) in Central Highlands were evaluated using eight SSR markers. Based on the SSR data, 21 alleles were detected by eight SSR with high polymorphism. The genetic diversity of levels within the populations were moderately high (Ho = 0.555, He = 0.429). The average number of shannon information index were 0.618 and genetic differentiation among populations was low (Fst=0.097). The AMOVA revealed high genetic variation within individuals (87%) compared among populations (13%). The UPGMA phenogram showed that the results of molecular clustering largely agreed with the pedigree and geographic origin. Three populations (NL, KCR and BDNB) were clustered together and CYS population was separated. The maximum quantity ΔK was observed for K=2 in population structure analysis, indicating that the entire collection could be divided into two main groups of genes. This study can provide a theoretical basis for genetic resource management and varieties identification of D. elatum resources, and provide reference basis for breeding.

Highlights

Dacrydium elatum (Roxb.) Wall. genus Dacrydium (Podocarpaceae) isconifer species in Vietnam, grows in primary closed evergreen seasonal forest tropical mixed they have been extensively used for furniture, handicrafts and construction [13]
Previous studies investigated the genetic variation and verified the taxonomic status of the Dacrydium species at the molecular level, such as D. pectinatum [14], D. imbricatus [15], D. elatum [16], the genetic structure of D. elatum has not been explored in Vietnam
All of SSR markers were polymorphic in four D. elatum populations

Summary

Introduction

Dacrydium elatum (Roxb.) Wall. genus Dacrydium (Podocarpaceae) isconifer species in Vietnam, grows in primary closed evergreen seasonal forest tropical mixed (elevations 700–2000m) they have been extensively used for furniture, handicrafts and construction [13]. Many populations of D. elatum are endangered by over exploitation and deforestation and we have serious lack of information on ecological characteristics and genetic variation at population and species levels of D. elatum, especially the negative effects of human activities. This situation urged to undertake a study to address these problem and to provide additional information for the Forest Protection Department in order to stress the need for the conservation and sustainable use of the species of local communities. It is a dire need of the day to explore the diversity and population structure of D. elatum and set breeding and conservation strategies

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