Genetic Structure, Differentiation and Originality of Pinus sylvestris L. Populations in the East of the East European Plain

Yulia Vasilyeva,Nina Pystogova,Nikita Chertov,Svetlana Boronnikova,Ruslan Kalendar,Yulia Nechaeva,Yana Sboeva

doi:10.3390/f12080999

Yulia Vasilyeva, Nina Pystogova + Show 5 more

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https://doi.org/10.3390/f12080999

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Abstract

In order to carry out activities aimed at conservation and rational use of forest resources; it is necessary to study the main forest-forming plant species in detail. Scots pine (Pinus sylvestris L., Pinaceae) is mainly found in the boreal forests of Eurasia and is not so often encountered in the east of the East European Plain. The aim of the study was to study the genetic diversity, structure and differentiation of Scots pine populations in the east of the East European Plain. We studied ten populations of P. sylvestris using the Inter Simple Sequence Repeats (ISSR)-based DNA polymorphism detection method. Natural populations are demonstrated by relatively high rates of genetic diversity (He = 0.167; ne = 1.279; I = 0.253). At the same time, there is a tendency for a decrease in the genetic diversity of the studied populations of P. sylvestris from west to east. Analysis of the genetic structure shows that the studied populations are highly differentiated (GST = 0.439), the intrapopulation component accounts for about 56% of the genetic diversity. Using various algorithms for determining the spatial genetic structure, it is found that the studied populations form two groups of populations in accordance with geographic location. With the help of a genetic originality coefficient, populations with specific and typical gene pools are identified. They are recommended as sources of genetic diversity and reserves for the conservation of genetic resources of the species.

Highlights

132 PCR amplicons were produced from five Inter Simple Sequence Repeats (ISSR) primers with an average of 26 highly polymorphic amplicons in P. sylvestris in the east of the Eastern
Our results on ISSR profiles in P. sylvestris are in agreement with previous work [34]
As a result of the analysis, a high level of genetic diversity is revealed in P. sylvestris populations, which is consistent with the data obtained earlier by Vidyakin et al [35] in the northeast of the Russian plain

Summary

Introduction

The study of genetic diversity, determination of the spatial and genetic structure, intra- and interspecific differentiation of coniferous species which are of great biosphere and resource importance make up one of the important tasks of population biology [3]. Scots pine (Pinus sylvestris L.; Pinaceae), one of the most widespread economically important forest-forming species, plays an extremely important role in the formation of the structure and functions of forest ecosystems [4,5,6]. The wood of this plant has many beneficial qualities and is versatile in its use. Investigations of the genetic diversity and differentiation of P. sylvestris populations are important for studying the

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Conclusion