Abstract
Genetic stability is highly important in terms of endangered species gene banks. Culture and cold stresses under in vitro conditions may lead to genetic variability. Many methods and possibilities to design appropriate starters for DNA-fingerprinting purposes increase cost and time consumption. Furthermore, multiplicity of possible primers makes it difficult to standardize plant research. The aim of this study was to verify effectiveness of various methods in assessing the clonal homogeneity of Taraxacum pieninicum plantlets regenerated after long-term in vitro cold-storage and to simplify the selection of the genetic stability analysis for Asteraceae family. Inter Simple Sequence Repeats (ISSR) and Start Codon Targeted (SCoT) polymorphism assays were performed to detect DNA sequence variation. No differences were observed using 16 ISSR markers and 12 SCoT markers between regrown plantlets after storage and plants cultivated from seeds in a soil. Furthermore, SCoT markers were most effective for screening T. pieninicum genome and appeared to be highly useful for different micropropagated and endangered species of Asteraceae family.
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