Genetic stability analysis and molecular depiction in elite entries of rice (Oryza sativa L.)

Magdy Ahmad Al-Kordy,Hayam Fouad Ibrahim,Almoataz Bellah Ali El-Mouhamady,Hamdy Mahmoud Abdel-Rahman

doi:10.1186/s42269-019-0100-3

Magdy Ahmad Al-Kordy, Hayam Fouad Ibrahim + Show 2 more

Open Access

https://doi.org/10.1186/s42269-019-0100-3

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Abstract

BackgroundGenetic stability is considered one of the most important genetic tests used to ascertain the extent of genetic stability reached in plants; consequently, the goal of this research is to detect the degree of genetic stability of a group of a superior rice lines under different climatic environments.ResultsThe seven entries of rice exhibited highly genetic stability depending on the results obtained from genetic stability analysis where they were recorded as high yielding; in addition, positive data for the remaining traits studied under the 12 environmental conditions were tested. Line numbers 1, 3, 4, and 5 were in the first rank for high genetic stability and high stable yielding under all experiments, while line numbers 2, 6, and 7 were recorded in the second rank. The values of broad sense heritability were very high in some traits (plant height, heading date, number of filled grains/panicle, grain yield/plant, and flag leaf area) which indicated that the genetic variance played an important role for controlling and inheriting these traits. A total of 101 fragments were generated using six primers of ISSR through comparison among the seven rice lines, where 34 of them were monomorphic and 67 bands were polymorphic with 66.33% polymorphism.ConclusionFrom the previous results, it could be concluded that the seven promising lines showed high genetic stability and recorded highly stable yielding under various environments which confirmed their importance in rice breeding programs for enhancing salinity tolerance, resistance to many diseases, and other stresses under Egyptian conditions.

Highlights

Genetic stability is considered one of the most important genetic tests used to ascertain the extent of genetic stability reached in plants; the goal of this research is to detect the degree of genetic stability of a group of a superior rice lines under different climatic environments
The impact of water stress on six promising lines of rice and their F1 crosses were performed by Eldessouky et al (2016) who tested these materials under wet and drought conditions as well as comparing them using seven various primers from Inter-Simple Sequence Repeat Markers (ISSR) and showed that the seven primers used in this investigation generated 52 fragments, 37 of which were polymorphic with polymorphism (71.15%), while the rest of bands were monomorphic
Heiba et al (2016) exhibited the effect of some levels of heavy metals on yield and its trait components in rice genotypes using RAPD-polymerase chain reaction (PCR) markers to compare the rice entries under study and the results exhibited that the entry genotypes (Sakha 106, GZ842312-9-4-5-1,IR 75, Giza 179 X IR 60, and GZ842312-9-4-5-1X IR 75) were recorded as highly tolerant for increasing the rates of heavy metals in soil as well the position of multi-environment treatises and its effect used for enhancing the resumption of rice yield toward diversified ambiences

Summary

Introduction

Genetic stability is considered one of the most important genetic tests used to ascertain the extent of genetic stability reached in plants; the goal of this research is to detect the degree of genetic stability of a group of a superior rice lines under different climatic environments. The rendering of rice genotypes in phlegmatic conditions was reported by Nanculao et al (2016), estimating six rice entries beside four empirical genotypes within three regions over three years They confirmed that the entry Quila 241319 is considered the preferable empirical genotype and was recorded as high yielding (11.3 t ha-1) as well as detecting high production stability using the environments; mercantile varieties were identified as moderate grain yield entries. The main objective of this study is to ascertain the degree of genetic stability and environmental acclimatization for the seven rice entries being studied In the past, these lines have shown a high tolerance to salinity and a high level of heavy metals in various regions beside different kinds of diseases and the test of water stress resistance will come in future years

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