Abstract
The gene df (deformed flower), which is expressed as short anther in sensitive cytoplasm, has been incorporated into the trisomic series of S. chacoenseBitt. The cytoplasmic sensitivity and genotypes for the gene df were tested in the original trisomics and their parents. Two diploid parents were found to be homozygous dominant for Df, while one was homozygous recessive. All the parents and trisomics tested had resistant cytoplasm. Six trisomics were heterozygous for df, segregating with a 1:1 ratio when they were crossed to the homozygous testers ([dfs]dfdf). When the F1 trisomics (trisomics x [Dfr]dfdf) were crossed to the homozygous testers, nine of the 13 trisomics segregated with a 1:1 ratio, three with a 0:1 ratio (all deformed) and one trisomic (V1682.3) with a 2:1 ratio. All the parents of the trisomic V1682.3 were homozygous dominant for Df. This clone is believed to be trisomic for the gene df.
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