Genetic screening for in vivo modifiers of Arp-like35E allele phenotype from Drosophila melanogaster.

Abstract

According to the two-hybrid–based protein-interaction map of the fruit fly proteome the principal interactor of ARP-like is endophilinA. Taking advantage of two different transgenic strains having in the lab, which contain differently loss-of-function lethal (LoF) mutations of these genes, ARP-like35E and respectively, endoAEP927, it was generated a new stock bearing recombinant chromosomes carrying both LoF mutations. The goal was to test if the assumed interaction between both wild-type alleles is also taken place between their LoF alleles but in Drosophila not in yeast, in vivo, in the context of an intact organism. Preliminary comparative analyzes of the GFP and non-GFP larvae have shown that the phenotype of the double-mutant combination, ARP-like35E_endoAEP927 was aggravated. The double-mutants died earlier than the single mutants taken separately, which suggested that some interaction could have been really taken place between the allele’s products. Due to the fact that both of the considered genes are orthologous to human genes implicated in different pathologies, in fact, this study represents a valuable source of novel protein-protein interaction with relevance to human diseases.