Abstract

Yardlong bean is a very popular vegetable with moderately high nutritional value and versatile. The genetic relationships among yardlong beans should be explored for long-term success in its breeding programs. The aim of the study was to evaluate genetic relationships among yardlong bean F1 crossbred progenies and their parents based on DNA markers. RAPD was performed on five genotypes of F1 yardlong bean progenies from diallelic crosses and three cultivars of parental plants. Five selected primers generated 16 scorable bands with their size ranging from 280 bp to 1160 bp, and the average number of bands per primer of 3.20. Cluster analyses using UPGMA procedure were performed using MVSP 3.1 software and produced a dendrogram with two clusters. Jaccard similarity matrices among yardlong bean parents and their F1 progenies varied between 0.40 – 0.73. The results suggest that RAPD markers can be an efficient, fast, and inexpensive way for differentiation of yardlong bean F1 progenies and their parents, as well as to evaluate their genetic relationships.

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