Abstract

SummaryStarch gel‐electrophoresis was used to assess genetic differences between two populations of Barbus motebensis and two other taxonomic related and morphologically similar barb species. Ten of the 29 protein coding loci studied were polymorphic. In spite of the small bio‐metric and meristic differences between the species studied, the extent of genetic differences suggests four distinct species. The evidence in support of a new barb species in the Ohrigstad River further adds to the current conservation importance of the rivers of the eastern Transvaal escarpment as a ‘hotspot’ for endemic fish species.

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