Abstract
Genetic relationships among the violet root rot fungi were studied by hyphal anastomosis and sequencing of the ITS-5.8S rDNA regions. Twelve isolates of Helicobasidium mompa , one isolate each of H. purpreum and H. compactum , two isolates of Helicobasidium sp., eight isolates of Rhizoctonia crocorum , and three isolates of R. violacea anastomosed in most combinations, resulting in the death of fused cells and adjacent cells. Phylogenetic analyses of the ITS-5.8S rDNA regions separated 31 violet root rot fungal isolates into five lineages: H. mompa from Japan and Korea; R. crocorum from Europe and New Zealand; H. purpureum, R. crocorum , and R. violacea from Europe; Helicobasidium sp. from Japan; and H. compactum from the USA. Within each lineage there was no more than one nucleotide substitution, and similarity ranged from 81.8% to 98.2% between lineages. The discrepancy between the results from the two methods are discussed in terms of the biological species concept in the complex.
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