Genetic Relationship of Ornamental Peach Determined Using AFLP Markers

Abstract

Ornamental peach (Prunus persica (L.) Batsch) is a popular plant for urban landscapes and gardens. However, the genetic relationship among ornamental peach cultivars is unclear. In this report, a group of 51 ornamental peach taxa, originated from P. persica and P. davidiana (Carr.) Franch., has been studied using AFLPs. The samples were collected from China, Japan, and US. A total of 275 useful markers ranging in size from 75 to 500 base pairs were generated using six EcoRI/MseI AFLP primer pairs. Among them, 265 bands were polymorphic. Total markers for each taxon ranged from 90 to 140 with an average of 120. Two clades were apparent on the PAUP–UPGMA tree with P. davidiana forming an outgroup to P. persica, indicates that P. davidiana contributed less to the ornamental peach gene pools. Within P. persica clade, 18 out of 20 upright ornamental peach cultivars formed a clade, which indicated that cultivars with upright growth habit had close genetic relationship. Five dwarf cultivars were grouped to one clade, supported by 81% bootstrap value, indicating that they probably derived from a common gene pool. These results demonstrated that AFLP markers are powerful for determining genetic relationships in ornamental peach. The genetic relationships among ornamental cultivars established in this study could be useful in ornamental peach identification, conservation, and breeding.