Genetic Relationship of Hibiscus spp. Based on DNA bands Using RAPD Technique

Abstract

The variety of Hibiscus can be categorized based on genetic relationship. This research aimed to figure out genetic relationship of Hibiscus spp. (Hibiscus rosa-sinensis (Hrs), Hibiscus schizopetalus (Hs), and Hibiscus tiliaceus (Ht)) based on DNA bands using Random Amplified Polymorphic DNA technique and find out the band of DNA to categorize Hibiscus spp. in dendrogram. The leaves of the samples were collected from Kebun Raya Purwodadi – Pasurusan, Indonesia and then used as DNA sources. The primer used in this study were OPA-1, OPA02, and OPA-3. The experiment resulted 56 bands of DNA which were obtained from amplification process. Subsequently, we performed cluster analysis by applying Unweighted Pair Group Method with Arithmetic Mean (UPGMA), in which simple matching, and Principal Component Analysis (PCA) analysis were selected to obtain dendrogram. The resulted dendrogram exhibited that H. rosa-sinensis possess a closed genetic relationship (index similarity 0.65) with H. schizopetalus but showed far genetic relationship with H. tiliaceus (index similarity 0.40)