Genetic regulation of chromosome behaviour in interspecific hybrids of Drosophila

Abstract

When crossing Drosophila virilis females with D. littoralis males, the elimination of D. littoralis sixth chromosome (microchromosomes) was often observed. The absence of the sixth chromosome of D. littoralis was revealed when studying F1 hybrids, because of the mosaic expression of the recessive gene "gl", located in the sixth chromosome of D. virilis. In the reciprocal cross the elimination of the sixth chromosome of D. littoralis did not take place (Sokolov 1959).Genetic analysis enabled the authors to conclude that the observed maternal effect on mitosis is controlled by recessive genes located on the second and fourth chromosome of D. virilis. The genes located on the second chromosome, differ from those on the fourth chromosome both in temperature sensitivity and in the time and/ or the mechanism controlling the mitotic behaviour of the chromosomes.By means of back-crosses a new stock was established where all chromosomes except the sixth belonged to D. virilis. The sixth pair (microchromosomes) in this line was represented by one D. virilis and one D. littoralis chromosome. It was shown that the sixth chromosome of D. littoralis might be eliminated or undergo non-disjunction in D. virilis germline but the frequency of such atypical behaviour was very low (about 2 %). Low temperature treatment was not effective for increasing the frequency of either elimination or non-disjunction of the D. littoralis sixth chromosome in D. virilis germ-line.