Abstract
Ultrathin-layer isoelectric focusing of seed proteins was used to test the genetic purity of pumpkin hybrid seeds. In the pH range of 5-8, one male marker band and two female marker bands were found in the male and female parental line, respectively. Based on the male marker band and female marker bands, the genetic purity of F1 seeds can easily be determined in the laboratory.
Highlights
Pumpkins (Cucurbita moschata Duch.) are widely grown for commercial use in food and recreation
The grow out test (GOT) for genetic purity of pumpkin hybrid seeds has traditionally been conducted and is based on morphological criteria that can be influenced by environmental conditions
The aim of the present study was to investigate the applicability of ultrathin-layer isoelectric focusing (UTLIEF) for testing the genetic purity of pumpkin hybrid seeds
Summary
Pumpkins (Cucurbita moschata Duch.) are widely grown for commercial use in food and recreation. Most pumpkin seeds are of F1 hybrid cultivars due to their vigor, uniformity, disease resistance, stress tolerance and good horticultural traits. To assure the uniformity and stability of field performance and yield, it is necessary to examine the genetic purity of commercial hybrid pumpkins prior to sale. The grow out test (GOT) for genetic purity of pumpkin hybrid seeds has traditionally been conducted and is based on morphological criteria that can be influenced by environmental conditions. GOT is limited by the accuracy required, time and labor cost (Cooke 1995). Isozyme analysis can be used for determination of genetic purity. This method is limited due to many factors affecting isozyme expression, including development of plant tissue and the environment. Less loci and restricted polymorphism may affect the utility of these markers (Tanksley and Jone 1981, Arús et al 1985)
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