Abstract
ObjectiveTo determine if genetic polymorphism of VEGF is associated with the development of endometriosis in Nigerian women.Study designCase control study of 100 women (50 healthy controls and 50 with endometriosis). Serum VEGF concentration of participants were determined using enzyme-linked immunosorbent assay (ELISA) technique. Genomic DNAs were isolated from peripheral blood samples and quantified by nanodrop spectrophotometer one. Single nucleotide polymorphisms genotyping was carried out by polymerase chain reaction and restriction fragment length polymorphism (PCR–RFLP).ResultsMean age of participants was 32.96 ± 6.91 years for control and 32.04 ± 7.56 years for cases. VEGF levels in case and control groups were not statistically different (82.68 pg/ml [69.11–121.11 pg/ml] vs. 82.81 pg/ml [72.90–113.82 pg/ml] respectively; p = 0.967). All four genotypes examined were in Hardy–Weinberg equilibrium. Minor allele frequency of − 460T > C, − 1154G > A, + 936C > T and + 2578C > A were 24%, 8%, 6% and 10% in the control and 19%, 9%, 5% and 14% in endometriosis patients. However, allele and genotype distributions of − 460T > C, − 1154G > A, + 936C > T and + 2578C > A VEGF polymorphisms in endometriosis patients and control were not significantly different (p > 0.05).ConclusionOur preliminary findings revealed no association between endometriosis and − 460T > C, − 1154G > A, + 936C > T and + 2578C > A of VEGF genes among Nigerian women.
Highlights
Endometriosis is defined as the presence of endometrial glands and stroma at sites other than the uterine cavity
Our preliminary findings revealed no association between endometriosis and − 460T > C, − 1154G > A, + 936C > T and + 2578C > A of VEGF genes among Nigerian women
Study population Consenting women with severe endometriosis as defined by the revised American Society of Reproductive Medicine Classification of Endometriosis [21] were recruited from the Gynaecology out-patient clinics, Accident and Emergency (A&E) unit and Gynaecological wards of the hospital as cases, while women who did not have endometriosis were recruited as controls
Summary
Endometriosis is defined as the presence of endometrial glands and stroma at sites other than the uterine cavity. Endometriosis affects 0.8– 28.6% of women of reproductive age [1, 2]. It is a common cause of chronic pelvic pain, with a prevalence of. The role of genetics in endometriosis has been studied over time and there appears to be a genetic predisposition in certain individuals by ancestry [5,6,7]. It has been found that African American women appear to have a predilection for uterine implants of endometriosis, and this
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