Genetic polymorphisms of MiR-499a (rs3746444) and MiR-196a2 (rs11614913) in ischemic stroke and correlation with risk factors

Abstract

miRNAs have functional roles in ischemic stroke through regulating their target genes. The present study aimed to assess the association between the genetic polymorphisms of miR-499a A>G (rs3746444) and miR-196a2 T>C (rs11614913) and the risk factors of ischemic stroke.With respect to miR-499a A>G, the AG genotype was associated with an increased risk of ischemic stroke (p = 0.01). However, the AA genotype had a significant protective role for ischemic stroke among females (p = 0.03). No significant association was found between different genotypes and such risk factors as diabetes mellitus, hypertension, and aging.The TT genotype of miR-196a2 T>C had a significant protective role (OR = 0.4, 95 % CI = 0.2–0.9, p = 0.02). Additionally, the CC genotype and C allele of miR-196a2 T > C significantly increased the risk of ischemic stroke (OR = 2.4, 95 % CI = 1.3–4.5, p = 0.005 and OR = 1.9, 95 % CI = 1.3–3.01, p = 0.001, respectively). However, the inheritance of C allele had a protective role against diabetes (p = 0.01). No association was observed between the miR-196a2 genotypes and hypertension, gender, and age.The association between both miR-499a and miR-196a2 genetic polymorphisms and the pathogenesis of ischemic stroke indicated that these polymorphisms could be used as potential biomarkers for the susceptibility of ischemic stroke.