Genetic polymorphisms of glutathione S-transferase M1 and T1, and evaluation of oxidative stress in patients with non-small cell lung cancer.

Hongyan Zhang,Kaifa Tang,Zhidong Li,Xuwei Wu,Yi Xiao,Xing Wei,Mei Chen

doi:10.1186/s40001-014-0067-3

Abstract

BackgroundOur objective is to investigate the genetic polymorphisms of the glutathione S-transferase M1 and T1 genes (GSTM1 and GSTT1) and evaluate oxidative damage in patients with non-small lung cancer (N-SCLC).MethodsOne hundred and ten patients with N-SCLC and 100 controls are included in this case-control study. Multiplex polymerase chain reaction (PCR) analyses were used to identify the genotypes. The activities of malondialdehyde (MDA) and nitric oxide (NO) and total antioxidant capacity (T-AOC) were detected by spectroscopic analysis.ResultsThe frequencies of the GSTM1, T1, and GSTM1/T1 null genotypes in the patient group were significantly higher than that in the control group (OR = 2.071, P = 0.009; OR = 1.900, P = 0.024; OR = 3.258, P = 0.003). The activities of MDA and NO were significantly higher in the patient group than that in the control group (P <0.001), and T-AOC was significantly lower in patient group than that in control group (P <0.001). The activities of MDA, and NO were higher but the T-AOC was lower in patients with the GSTM1, T1 and M1/T1 null genotypes than those in patients with GSTM1, T1 and M1/T1 present genotypes (P <0.001).ConclusionsOur results suggest that oxidative damage may be play a important role in patients with N-SCLC, and that GSTM1 and GSTT1 null genotypes may predispose the cells of patients with N-SCLC to increased oxidative damage.

Highlights

Our objective is to investigate the genetic polymorphisms of the glutathione S-transferase M1 and T1 genes (GSTM1 and glutathione S-transferases T1 (GSTT1)) and evaluate oxidative damage in patients with non-small lung cancer (N-SCLC)
The frequency of the GSTM1 null genotype was 42.0% in the control group and 60.0% in the patient group (OR = 2.071; confidence intervals (CI) 95%, 1.194 to 3.593; P = 0.009), and 34.0% and 78.0% (OR = 4.132; CI 95%, 1.957 to 8.724; P
Our results suggest that the levels of MDA and nitric oxide (NO) were higher in the patient group than that in the control group, but the level of total antioxidant capacity (T-antioxidant capacity (AOC)) was lower in the patient group than that in the control group

Summary

Introduction

Our objective is to investigate the genetic polymorphisms of the glutathione S-transferase M1 and T1 genes (GSTM1 and GSTT1) and evaluate oxidative damage in patients with non-small lung cancer (N-SCLC). Previous study has shown that oxidative stress and free radicals have been associated with an increased risk of various cancers [5]. Demonstrated that low antioxidant capacity (AOC) in those who never smoked but that have N-SCLC may have contributed to excessive oxidative DNA damage in the lung tissues [8]. Peddireddy et al showed that an evidencebased increased rate of oxidative stress plays a role in the pathogenesis of N-SCLC because a failure in the oxidant/antioxidant balance favors lipid peroxidation and DNA damage [9]

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