Abstract
BackgroundChemokines are well known inflammatory factors critical for tumor development in diverse tissues, including lung cancer. Chemokine (C-C motif) Ligand 2 (CCL2) was one of such chemokines important for both primary tumor development and metastasis of various cancers. Polymorphism at rs3760396 of CCL2 genes is associated with the prognosis of non-small cell lung cancer (NSCLC). The goal of our study was to examine the relationship of genetic polymorphisms rs3760396 with the susceptibility of lung cancer and its pathological subtypes in Han-ancestry Chinese population.Methodsrs3760396 G/C polymorphism of CCL2 was genotyped using PCR in 394 patients with lung cancer and 545 cancer-free controls from the same Northeast region of China.ResultsAfter controlling for gender, age and smoking status, no significant association was observed between rs3760396 polymorphism and overall lung cancer. However, minor allele G of rs3760396 polymorphism was significantly associated with increased risk of adenosquamous lung carcinoma with either allelic genetic model (OR = 5.29, P < 0.001), or dominant genetic model (OR = 9.88, P < 0.001), or genotypic model (GC genotype vs. CC genotype, OR = 10.73, P < 0.001). Although rs3760396 polymorphism was not significantly associated with increased risk of adenocarcinoma subtype, it was nominally associated with the pooled outcome of either adenocarcinoma or adenosquamous carcinoma under allelic genetic model (OR = 1.54, P = 0.023) or dominant genetic model (OR = 1.57, P = 0.031).ConclusionsOur study suggested rs3760396 polymorphism of CCL2 is associated not only with prognosis of NSCLC, but also with risk of lung cancer in a subtype-specific manner. Our results further supported previous evidence of the important role of CCL2 in lung cancer development.
Highlights
Chemokines are well known inflammatory factors critical for tumor development in diverse tissues, including lung cancer
We summarized genotype distribution and allele frequency of rs3760396 single nucleotide polymorphism (SNP) in chemokine ligand 2 (CCL2) gene in controls and lung cancer cases (Table 2). rs3760396 SNP genotype distribution in both cases and controls was in agreement with the Hardy-Weinberg equilibrium (p = 0.40 and 0.53, respectively)
We found that rs3760396 SNP was significantly associated with adenosquamous carcinoma using either allelic (OR = 5.3 and p < 0.001), dominant (OR = 9.8 and P < 0.001), or genotypic model (OR = 10.7 and P < 0.001 for GC vs. CC genotypes) even under conservative Bonferroni correction (p value cutoff 0.0017)
Summary
Chemokines are well known inflammatory factors critical for tumor development in diverse tissues, including lung cancer. Polymorphism at rs3760396 of CCL2 genes is associated with the prognosis of non-small cell lung cancer (NSCLC). Most lung cancer patients are diagnosed at later stage when the timing for effective surgical dissection is missed. It has been found that both genetic and environmental factors are critical for the development of lung cancer. Identification of genetic risk factors such as genetic polymorphism can have important indication for both early detection and therapy target discovery to improve the prognosis of lung cancer patients. Recent research has successfully identified potential genetic variations associated with the susceptibility of lung Cancer [5,6,7,8]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
