Genetic polymorphism of human platlet antigen-17 alloantigen system in Heilongjiang Manchu population

Abstract

Objective To analyze the allele frequencies and its polymorphisms of human platlet antigen(HPA) 1 to 17 system in Heilongjiang Manchu population for preparation of setting up HPA genotype data bank. Method A total of 101 samples from healthy voluntary platelet donors in Heilongjiang were genotyped for HPA-1 to 17 by PCR-SSP and the genotype frequency and gene frequency were calculated. Result : The gene frequencies of HPA-1a to 17 were 0.99505, 0.940595, 0.549505, 1.0, 0.99505, 0.9802, 1.0, 1.0, 1.0, 1.0, 1.0, 1.0, 1.0, 1.0, 0.445545, 1.0, and 1.0 respectively.The gene frequencies of HPA-1b to -3b and -5b to -6b and 15b were 0.00495, 0.059405, 0.450495, 0.00495, 0.0198, and 0.554455 respectively.HPA-4b and 7b to 14b and 16b to 17b were not detected.The study for the first time investigated the assortment of HPA genes and their frequencies.There were 22 assortment in Heilongjiang Manchu population, only 3 assortment of HPA gene frequencies more than 10%, 19 assortment of HPA gene frequencies less than 10%.HPA-3 frequency of Manchu were significantly different from that Han population in Heilongjiang(P<0.05). Conclusion Distribution of the allele polymorphism of HPA 1-17 in Heilongjiang Han population is similar to that in Manchu population, and it exhibits its own characteristic.The system of HPA-3, 15 in Manchu population are polymorphic and it is the key point of HPA suitable infusion.The incompatibility rates of donor recipient HPA-3, 15 were 37.25% and 37.20% in random HPA infusion. Key words: Human platelet antigens; Ethnic minorities; Genotype