Abstract
Electrophoresis of rainbow trout plasma proteins in agarose gel yields three bands that react with a monoclonal antibody (MoAb) to trout complement component C3. The bands are visualised by an immunoblotting procedure in which the separated proteins are transferred to nitrocellulose paper, followed by staining of the C3-bands with the MoAb. When individual fish were analysed, one or two bands could be detected in each plasma sample. In the population studied, a total of three distinctive bands were found, which were named according to their electrophoretic mobility towards the anode: slow( s ), fast 1 ( f 1 ) and fast 2 ( f 2 ). Two rainbow trout families and a number of individual samples have been screened, and a genetic basis for the polymorphism has been established.
Published Version
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