Genetic Polymorphism and Clinical Significance of Human Platelet Antigen-2 in Platelet Membrane Glycoprotein I bα of Yao Minority Population in Guangxi Zhuang Autonomous Region

Abstract

Objective To investigate the genetic polymorphism and clinical significance of human platelet antigen (HPA)-2 in platelet membrane glycoprotein (GP) Ⅰ bα in Yao minority population of Guangxi Zhuang Autonomous Region.Methods From March 2009 to May 2010,a total of 883 healthy adults from Yao minority in Guangxi Zhuang Autonomous Region were randomly selected into the study and their blood samples were collected.HPA genotypes of all the Yao minority population healthy adults were analyzed by polymerase chain reaction with sequence-specific primers (PCR-SSP).(Informed consent was obtained from all participants before collecting the blood samples.) Results Among the 883 blood samples,the genotype frequencies of HPA-2a/2a,HPA-2a/2b and HPA-2b/2b were 0.763 3,0.218 6 and 0.018 1 respectively.The gene frequencies of HPA-2a and HPA-2b were 0.872 6 and 0.127 4 which were similar to the frequencies reported in most European studies,but quite different from Chinese Han population and Southeast Asia population.Among the 883 blood samples,1 case of HPA-2b/2b 482T/T rare mutation was found.Conclusions The main characteristic of platelet GP Ⅰ bα polymorphisms of Yao minority population in Guangxi Zhuang Autonomous Region has now been defined in the study,which will be an important evidence for further studies to assess the role of platelet GP Ⅰ bα in individual predisposition to prothrombotic conditions and response to antithrombotic therapy.Data obtained in this study can be used for better understanding and treatment of immune-mediated platelet disorders in Guangxi Autonomous Region. Key words: Platelet membrane glycoprotein; Antigen, human platelet; Polymorphism, genetic; Yao minority