Abstract
In 1964 Mertz et al. reported that opaque-2 (O2) gene changed the protein composition and increased the lysine content of maize endosperm. A second high lysine maize mutant floury-2 (fl2) was identified and reported in 1965. Recently, it was found that several of starch-modifying genes increased the lysine content of maize endosperm substantially above the isogenic normal control, and each gene showed an enhanced effect on lysine when combined with O2 gene. Mutants with starch-modifying genes were concerned with changes influencing gelatinization temperature, viscosity, gel stability of starch, starch granule digestibility, and the production of amylose, amylopectin, water-soluble polysaccharides, and sugars. In studies of starch-modifying genes and their combination with O2 or fl2 gene, it was shown that O2 or fl2 dose not change the production of amylose, amylopectin, water-soluble polysaccharides, and sugars of maize kernels with each one of starch-modifying gene, and O2 gene has no effect on susceptibility of starches with amylases. It seems probable that the interaction of the O2 gene with many of starch mutants of the maize endosperm provide a variety of maize containing starch with characteristics specific for the starch mutant and protein with high nutritional quality.
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