Abstract
Cyclodextrin glycosyltransferase( CGTase,EC 2. 4. 1. 19) is an important enzyme with multiple functions,particularly the production of cyclodextrins( CD) and carbohydrate glycosylation. It is also applied in food industry. To modify the molecular structure of CGTase for improved performance in industrial applications,heterologous expression, site-directed mutagenesis and immobilization technology of cyclodextrin glycosyltransferase gene have been applied. Moreover,in recent years substantial progress has been made. In order to provide the reference in the related CGTase research,the heterologous expression strategies,the molecular engineering approaches,other technologies such as immobilized enzyme etc. were systematically summarized and discussed.
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