Abstract
We present a seminested PCR method that specifically discriminates between Plasmodium ovale curtisi and P. ovale wallikeri with high sensitivity. The test is based on species-specific amplification of a size-polymorphic fragment of the tryptophan-rich antigen gene, potra, which also permits discrimination of intraspecific sequence variants at this locus.
Highlights
We present a seminested PCR method that discriminates between Plasmodium ovale curtisi and P. ovale wallikeri with high sensitivity
Molecular-based detection revealed a dimorphism in the P. ovale A-type small subunit rRNA genes [6, 7], which extended to other genes [8]
Multilocus sequence analysis of isolates from diverse geographical origins culminated in the proposal that there were two species, P. ovale curtisi and P. ovale wallikeri [9]
Summary
We present a seminested PCR method that discriminates between Plasmodium ovale curtisi and P. ovale wallikeri with high sensitivity. This was exploited in a nested PCR detection assay [11], where primers target sequences conserved between these two genes and the species are discriminated by the size of the amplified fragments (299 bp or 317 bp for poctra; 245 bp for powtra).
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