Genetic mapping of QTLs for horticulture traits in a F2-3 population of bitter gourd (Momordica charantia L.)

Abstract

An extensive genetic linkage map was constructed for bitter gourd (Momordica charantia L.) via the study of F2 progenies derived from two cultivated inbred lines (gynoecia Z-1-4 and 189-4-1). The map included 194 loci on 11 chromosomes consisting of 26 EST-SSR loci, 28 SSR loci, 124 AFLP loci, and 16 SRAP loci. This map covered 1005.9 cM with 12 linkage groups. A total of 43 quantitative trait loci (QTLs), with a single QTL associated with 5.1–33.1 % phenotypic variance, were identified on nine chromosomes for 13 horticulture traits by analyzing the F2-3 families and the genetic linkage map. The 13 horticulture traits which were investigated in three environments included female flower ratios (FFR), first female flower node (FFFN), fruit length, fruit diameter, flesh thickness, fruit shape, fruit pedicel length, fruit length pedicel ratios, fruit weight (FW), fruit numbers per plant (FPP), yield per plant (YPP), stem diameter (SD), and internodes length (IL). One QTL cluster region was detected on Lg-5 which contained the most important QTLs for YPP, FPP, FFFN, FFR, and FW with high contributions to phenotypic variance (5.8–25.4 %).