Abstract
AbstractSeveral molecular genetic approaches are available for identifying the location of a conserved human DNA sequence in the mouse genome. The choice of approach depends on the reagents available and the precise information that the investigator would like to determine. This unit presents a method for determining the location of the murine homologue of a human gene using linkage analysis with available mouse interspecific backcrosses. Restriction fragment length polymorphism (RFLP) analysis is used to map human gene fragments in the mouse genome. The approaches described in this unit are analogous to those used for linkage analysis in human pedigrees, except the mouse crosses can and have been designed so only two alleles are segregating for each locus and the phase of every chromosome is known.
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