Genetic mapping and validation of QTLs associated with resistance to Calonectria leaf blight caused by Calonectria pteridis in Eucalyptus

Abstract

Calonectria leaf blight (CLB) caused by Calonectria pteridis is one of the main leaf diseases in Brazilian Eucalyptus plantations in warm climates with prolonged periods of rain. The main symptoms are leaf spots followed by intense defoliation in highly susceptible plants. Exploiting the existing inter- and intraspecific variability for defoliation is the best option to control this disease. Nevertheless, nothing is known about the genetic architecture of resistance to CLB in Eucalyptus. We built microsatellite- based genetic maps for E. urophylla×E. camaldulensis (EU11×EC06) F1 family of 89 plants. Four or five clonal replicates per individual offspring were clonally propagated, totalling 445 plants which were phenotyped for defoliation in the basal third of the branches at 30 days following controlled inoculation with a single-spore isolate. Genetic mapping was performed using a pseudo-testcross, and QTLs detected using composite interval mapping. Five QTLs were detected for resistance to CLB; of them, only one could be validated in two unrelated pedigrees, and its effect was conservatively estimatedascontrollingbetween5and10%ofthephenotypic variation when the bias derived from the limited size of the mapping population was taken into account. This work pro- vides a starting point for future studies of the genetics of resistance to CLB, and adds further evidence to the challenge of ascertaining the effects of QTLs detected in a single bipa- rental background across unrelated families.