Abstract
Cold-induced sweetening (CIS) caused by reducing sugar (RS) accumulation during storage in low temperature in potato tubers is a critical factor influencing the quality of fried potato products. The reconditioning (REC) by arising storage temperature is a common measure to lower down RS. However, both CIS and REC are genotype-dependent and the genetic basis remains uncertain. In the present study, with a diploid potato population with broad genetic background, four reproducible QTL of CIS and two of REC were resolved on chromosomes 5, 6, and 7 of the CIS-sensitive parent and chromosomes 5 and 11 of the CIS-resistant parent, respectively, implying that these two traits may be genetically independent. This hypothesis was also supported by the colocalization of two functional genes, a starch synthesis gene AGPS2 mapped in QTL CIS_E_07-1 and a starch hydrolysis gene GWD colocated with QTL REC_B_05-1. The cumulative effects of identified QTL were proved to contribute largely and stably to CIS and REC and confirmed with a natural population composed of a range of cultivars and breeding lines. The present research identified reproducible QTL for CIS and REC of potato in diverse conditions and elucidated for the first time their cumulative genetic effects, which provides theoretical bases and applicable means for tuber quality improvement.
Highlights
Potato (Solanum tuberosum L.), with a world production of about 377 million tons in 20161 play an important role in food security in the world as it can be consumed as both staple food and processed products
We find for the first time that cumulative effects across reproducible quantitative traits loci (QTL) play crucial roles in tuber cold-induced sweetening (CIS) and REC, these effects on CIS were validated in a natural population (NP) suggesting the potentials of the linked markers used for potato quality breeding
In order to dissect the genetic loci controlling reducing sugar (RS) related to the traits of CIS (RS after storage at 4◦C for 30 d) and REC (RS after storage at 4◦C for 30 d followed by 20 d of REC at 20◦C), the RS content was measured for all genotypes grown under five different environments (Supplementary Table S2)
Summary
Potato (Solanum tuberosum L.), with a world production of about 377 million tons in 20161 play an important role in food security in the world as it can be consumed as both staple food and processed products. Potato tubers are often stored in low temperature to reduce loss from sprouting, shrinking, and pathogenesis. Exposed to low temperature for a certain period. QTL, Cold-Induced Sweetening, Recondition usually results in an accumulation of reducing sugars (RSs) in the tubers, a phenomenon known as cold-induced sweetening (CIS). The RS content can fall back to a low level through reconditioning (REC) before processing by increasing the storage temperature to above 20◦C, which allows a partial RS conversion to starch, or to be used by respiration (Illeperuma et al, 1998). Recondition is costly and insufficient since the REC capacity varies among potato genotypes, possibly the cause for most varieties undesirable for frying though they have low RS content at harvest
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