Abstract
A genetic linkage map for blueberry has been constructed from over 60 RAPD (random amplified polymorphic DNA) markers that segregated 1:1 in a testcross population of about 40 plants. Prior to map construction, polymerase chain reaction conditions were optimized and RAPD marker reliability was confirmed. The mapping population was derived from a cross between diploid blueberry plants: Fl interspecific hybrid, `US388' (V. darrowi, `Fla4B' × V. elliottii, `Knight'), and another V. darrowi, `US799'. The map currently comprises 12 linkage groups corresponding to the basic blueberry chromosome number and covers a total genetic distance of over 800 cM, with a range of 2-30 cM between adjacent markers. Interestingly, a few pairs of marker loci behaved differently from both linked and unlinked loci, being found at a much higher frequency in the recombinant configuration than the parental configuration possibly suggesting selection for certain combinations of alleles.
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