Genetic interactions between DET1 and intermediate genes in Arabidopsis ABA signalling

Abstract

Seed germination is regulated positively by light and negatively by the dormancy-promoting phytohormone abscisic acid (ABA). DE-ETIOLATED 1 (DET1) is a negative regulator of light signalling in Arabidopsis thaliana. In contrast, the bZIP transcription factor LONG HYPOCOTYL 5 (HY5) is a positive regulator of light signalling. HY5 also positively regulates ABA signalling by promoting the expression of ABA INSENSITIVE 5 (ABI5), a dormancy promoting transcription factor. Here we show that germination in det1 mutants is sensitive to ABA. Double mutant analysis indicates that det1 ABA sensitive germination requires HY5 and ABI5. DET1 forms a complex with DAMAGED DNA BINDING protein 1A/B (DDB1A/B). Another DDB1 complex containing DWA1 and 2 (DWD hypersensitive to ABA 1/2) has also been shown to negatively regulate ABA response. Double mutant analysis indicates that DWA1, DWA2, DDB1A, and DDB1B are also required for the det1 ABA sensitive germination phenotype. We also examined water loss in adult plants and found that the det1 rapid water loss phenotype is independent of HY5, ABI5, DWA1, DWA2, and DDB1B. These findings provide insight into interactions between ABA and light signalling in Arabidopsis.