Abstract
BackgroundStress response is mediated by the transcription of stress-responsive genes. The F-box motif protein Saf1p is involved in SCF-E3 ligase mediated degradation of the adenine deaminase, Aah1p upon nutrient stress. The four transcription regulators, BUR6, MED6, SPT10, SUA7, are listed for SAF1 in the genome database of Saccharomyces cerevisiae. Here in this study, we carried out an in-silico analysis of gene expression and transcription factor databases to understand the regulation of SAF1 expression during stress for hypothesis and experimental analysis.ResultAn analysis of the GEO profile database indicated an increase in SAF1 expression when cells were treated with stress agents such as Clioquinol, Pterostilbene, Gentamicin, Hypoxia, Genotoxic, desiccation, and heat. The increase in expression of SAF1 during stress conditions correlated positively with the expression of RLM1, encoding the Rlm1p transcription factor. The expression of AAH1 encoding Aah1p, a Saf1p substrate for ubiquitination, appeared to be negatively correlated with the expression of RLM1 as revealed by an analysis of the Yeastract expression database. Based on analysis of expression profile and regulatory association of SAF1 and RLM1, we hypothesized that inactivation of both the genes together may contribute to stress tolerance. The experimental analysis of cellular growth response of cells lacking both SAF1 and RLM1 to selected stress agents such as cell wall and osmo-stressors, by spot assay indicated stress tolerance phenotype similar to parental strain however sensitivity to genotoxic and microtubule depolymerizing stress agents.ConclusionsBased on in-silico and experimental data we suggest that SAF1 and RLM1 both interact genetically in differential response to genotoxic and general stressors.
Highlights
The natural population of eukaryotic cells, mostly microorganisms remains non-dividing state and proliferate upon nutrients availability [1]
Based on in-silico and experimental data we suggest that SCF associated factor 1 (SAF1) and Resistance to lethality of MKK1P386 overexpression (RLM1) both interact genetically in differential response to genotoxic and general stressors
We studied the Gene Expression Omnibus profile database (GEO) for the expression status of SAF1 during stress conditions, and yeast transcription databases were investigated for the association of transcription factors with the SAF1 expression during stress
Summary
The natural population of eukaryotic cells, mostly microorganisms remains non-dividing state and proliferate upon nutrients availability [1]. The proliferation of Saccharomyces cerevisiae cells from in and out of the quiescence phase due to nutrients availability or stress. The SCFSaf1-E3 ligase in S.cerevisiae recruits Aah1p (adenine deaminase) for ubiquitin-mediated degradation upon nutrient deprivation condition [7]. The AAH1 expression is down regulated during the transition from proliferative state to quiescence state by Srb10p and Srb11p regulation at the post-transcriptional level of Aah1p requires SCFSaf1-E3 ligase activity. The F-box motif protein Saf1p is involved in SCF-E3 ligase mediated degradation of the adenine deaminase, Aah1p upon nutrient stress. The four transcription regulators, BUR6, MED6, SPT10, SUA7, are listed for SAF1 in the genome database of Saccharomyces cerevisiae. We carried out an in-silico analysis of gene expression and transcription factor databases to understand the regulation of SAF1 expression during stress for hypothesis and experimental analysis
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