Abstract
Here, we report full-length genome sequences of influenza pH1N1 viruses obtained prior to and after propagation in MDCK cells. Paired comparisons of the genomes showed that each strain acquired 1.0 to 18.8 mutations per genome per replication cycle, which corresponds to 0.5 to 5.8 mutations per virus proteome per replication cycle. Our analysis indicates that pH1N1 viruses accumulated adaptive mutations among others in response to propagation in cell culture. These results could be important for vaccine and drug-sensitivity surveillance studies, as well as for vaccine and antiviral drug development programs where cell cultures are used for influenza propagation.
Highlights
We report full-length genome sequences of influenza pH1N1 viruses obtained prior to and after propagation in MDCK cells
We assess the genome stability of 10 pH1N1 strains prior to and after propagation in MDCK cells
We sequenced genomes of pH1N1 strains from patient nasopharyngeal aspirates (NPA) and after four passages in MDCK cells as described elsewhere [1, 2]
Summary
We report full-length genome sequences of influenza pH1N1 viruses obtained prior to and after propagation in MDCK cells. Influenza viruses receive much public attention because they cause major pandemics and epidemics heavily impacting public health and economy. Prevention, and treatment programs are under development to improve control of influenza outbreaks. Such programs usually utilize cell culture for virus propagation.
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