Abstract
The present experiment was conducted to induce genetic variation using gamma irradiation and ethyl methanesulphonate mutagenesis and isolate and analyze the desirable mutant lines of Vicia faba L. var. Vikrant and PRT-12 in the M3 generation. The seeds of the mutant lines isolated from 3rd generation plants were analyzed for different parameters including total protein content, protein profiles and mineral content using Lowry method, Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and atomic absorption spectrophotometer, respectively. SDS-PAGE profile of mutants from variety Vikrant showed more polymorphism and expression of proteins than mutants isolated from variety PRT-12. Mutant seeds showed significantly higher protein content (0.18 to 1.29%) and mineral content (0.21 to 2.98 mg.g-1) compared to the controls. The mutants selected from variety Vikrant showed more increase in protein and mineral contents than the mutants selected from PRT-12. The selected mutant lines may serve as a valuable genetic material to breed for the improvement of protein and mineral contents in faba bean and other valuable crop plants.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
