Abstract
Pittosporum eriocarpum Royle, a medicinally important taxon, is endemic to Uttarakhand region of Himalaya. It has become endangered due to over-collection and the loss of habitats. As raising plants through seeds in this plant is problematic, a reliable protocol for micropropagation using nodal explants has been developed. High shoot regeneration (95%) occurred in MS medium augmented with BA 0.4mg/l in combination IBA 0.6mg/l. In vitro regenerated shoots were rooted in MS medium supplemented with three auxins, of which 0.6 mg/l indole butyric acid proved to be the best for rooting (90%) with maximum number of roots per shoot. Thereafter, rooted plants were hardened and nearly 73% of rooted shoots were successfully acclimatized and established in the field. Start codon targeted (SCoT), inter simple sequence repeats (ISSR) and random amplified polymorphic DNA (RAPD) markers were used to validate the genetic homogeneity amongst nine in vitro raised plantlets with mother plant. DNA fingerprints of in vitro regenerated plantlets displayed monomorphic bands similar to mother plant, indicating homogeneity among the micropropagated plants with donor mother plant. The similarity values were calculated based on SCoT, ISSR and RAPD profiles which ranged from 0.89 to 1.00, 0.91 to 1.00 and 0.95 to 1.00 respectively. The dendrograms generated through Unweighted Pair Group Method with arithmetic mean (UPGMA) analysis revealed 97% similarity amongst micropropagated plants with donor mother plant, thus confirming genetic homogeneity of micropropagated clones. This is the first report on micropropagation and genetic homogeneity assessment of P. eriocarpum. The protocol would be useful for the conservation and large scale production of P. eriocarpum to meet the demand for medicinal formulations and also for the re-introduction of in vitro grown plants in the suitable natural habitats to restore the populations.
Highlights
Pittosporum eriocarpum (Pittosporaceae), commonly known as Agni [1], is an endangered [2] and endemic medicinal tree species of Uttarakhand region occurring at an altitudinal range of PLOS ONE | DOI:10.1371/journal.pone.0159050 July 19, 2016Genetic Homogeneity Assessment of Pittosporum eriocarpum1000-2200m [3]
We evaluated the genetic homogeneity of the in vitro grown plants through Start codon targeted (SCoT), inter simple sequence repeats (ISSR) and random amplified polymorphic DNA (RAPD) markers
Present study reports a holistic and reliable procedure for in vitro regeneration, greenhouse acclimatization and field transfer of P. eriocarpum. This is the first successful attempt to establish a micropropagation protocol for this species by nodal explants, which will provide a alternative method for its conservation as well as supply of the material for commercial use
Summary
Pittosporum eriocarpum (Pittosporaceae), commonly known as Agni [1], is an endangered [2] and endemic medicinal tree species of Uttarakhand region occurring at an altitudinal range of PLOS ONE | DOI:10.1371/journal.pone.0159050 July 19, 2016Genetic Homogeneity Assessment of Pittosporum eriocarpum1000-2200m [3]. Pittosporum eriocarpum (Pittosporaceae), commonly known as Agni [1], is an endangered [2] and endemic medicinal tree species of Uttarakhand region occurring at an altitudinal range of PLOS ONE | DOI:10.1371/journal.pone.0159050. It is reported to occur mostly on the calcium rich rocky slopes in exposed sites [6]. It is a small evergreen tree up to 5m height with alternate or whorled leaves. Application of root paste provides relief in rheumatic swelling [3]. The tree is suitable for soil conservation and reclamation of degraded sites [4]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
