Abstract
Two-spotted spider mite (Tetranychus urticae C. L. Koch, 1836), is one of the most destructive herbivores of common bean. Very little is known about the diversity among resistant sources in this crop. The present study was conducted to characterize 22 resistant and susceptible common bean genotypes by 8 Simple Sequence Repeats (SSRs) and 8 Random Amplified Polymorphic DNA (RAPD) markers. These SSR and RAPD primers produced 100 % and 81.8 % polymorphic bands. Based on RAPD fingerprints and SSR profiles, pairwise genetic similarity ranged from 0.0 to 0.857 and from 0.125 to 1, respectively. The resistant and susceptible common bean accessions were grouped together in the dendrograms generated from RAPD and SSR clustering analyses. The results indicate that RAPD and SSR analysis could be successfully used for the estimation of genetic diversity among genotypes. SSR markers could group genotypes according to their resistibility and susceptibility to the spotted spider mite but RAPD could not. Therefore, the SSR markers can facilitate the development of resistant common bean cultivars through breeding programs against T. urticae.
Highlights
Common bean (Phaseolus vulgaris L.) is a widely distributed crop of considerable importance in many countries around the world (Tanyolac, 2013)
The Simple Sequence Repeats (SSRs) markers can facilitate the development of resistant common bean cultivars through breeding programs against T. urticae
The results of this study indicate that Random Amplified Polymorphic DNA (RAPD) and SSR analyses could be successfully used for estimation of genetic diversity among common bean genotypes
Summary
Common bean (Phaseolus vulgaris L.) is a widely distributed crop of considerable importance in many countries around the world (Tanyolac, 2013). Different types of markers such as morphological markers (Gepts et al, 1986; Singh et al, 1991; Pickersgill et al, 2005; De La Cruz et al, 2005), isozyme markers 2005), phaseolin types by electrophoretic profiles (Gepts et al, 1986; Pereira & Souza, 1992; Maciel et al, 1999; Solano, 2005), and molecular markers of Restriction Fragment Length Polymorphism (RFLP) (Pickersgill et al, 2005), Amplified Fragment Length Polymorphism (AFLP) (Maciel et al, 2003), the Random Amplified Polymorphic DNA (RAPD) (Beebe et al, 2000) and Inter Simple Sequence Repeats (ISSR) (De La Cruz et al, 2005) have been used to assess the genetic diversity in common bean. Phaseolus species possess several natural resistances, that is, constitutive and inducible defence mechanisms against herbivores (Edwards & Singh, 2006; Ballhorn, 2011)
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