Genetic diversity, virulence factors, and antimicrobial resistance of Listeria monocytogenes from food, livestock, and clinical samples between 2002 and 2019 in China

Abstract

Listeria monocytogenes remains a significant public health threat, leading to invasive listeriosis with severe manifestations (i.e. septicemia, meningitis, and abortion) and up to 30% of fatal cases. Here, we aimed to investigate genotypic diversity, virulence profiles, antimicrobial resistance patterns from a large and integrated population of L. monocytogenes isolates in China (n = 369), including food (n = 326), livestock (n = 25), and hospitalized humans (n = 18) over the years (2002–2019). PCR-based serogrouping showed the dominance of serogroup 1/2a-3a (37.4%) in food, 4a-4c (76%) in livestock, and 1/2a-3a (44.4%) in humans. Phylogenetic lineage analysis revealed the dominance of lineage II (63.4%) in food, lineage III (76%) in livestock, and lineage II (55.5%) in humans. Altogether, 369 isolates were grouped into 55 sequence types (STs) via multi-locus sequence typing (MLST), which belonged to 26 clonal complexes (CCs) and 17 singletons. Among various STs, ST9 (26%) was the most abundant in food, ST202 (76%) in livestock, and ST8 (16.6%) in humans. Overall, ST4/CC4, ST218/CC218, and ST619 isolates harbored both LIPI-3 and LIPI-4 genes subsets indicating their hypervirulence potential. Additionally, a low resistance was observed towards tetracycline (5.1%), erythromycin (3.2%), cotrimoxazole (2.9%), chloramphenicol (2.7%), gentamicin (2.4%), and ampicillin (2.1%). Collectively, detection of hypervirulent determinants and antimicrobial-resistant phenotype among Chinese isolates poses an alarming threat to food safety and public health, which requires a continued and enhanced surveillance system for further prevention of human listeriosis.