Abstract
Pathogenic bacteria are responsible for heavy mortality in both wild and cultured fish. Molecular characterization was carried out using Random Amplified Polymorphic DNAPolymerase Chain Reaction technique (RAPD-PCR), sensitivity to antibiotics of bacteria in Clarias gariepinus post juveniles sampled. Bacteria were isolated from the gut, gills and skin of the fish. Identification was done using the conventional culture-based method. Thirty bacteria isolates were selected and the DNAs were extracted using CTAB method, PCR amplification of the isolates was carried out using RAPD primer and five primers were used. Data collected were subjected to descriptive (mean and standard deviation) statistics. There were 63 polymorphic and 14 monomorphic markers generated from the five RAPD markers. The primers generated 77 alleles altogether. Out of the 10 antibiotics used, Cephalexin recorded the highest inhibition zone (33 mm) on one sample, Gentamicin on second sample had (30 mm). The least inhibition zone was recorded in Cotrimoxazole on the second sample with (8 mm), 70.5 % bacteria strains were susceptible to Gentamicin while two samples displayed 100 % resistance to all the antibiotics. This study concluded that there are pathogenic and opportunistic bacteria species in C. gariepinus which could be zoonotic. Keywords: bacteria isolates, genetic diversity, RAPD-PCR, DNA extraction, sensitivity, Clarias gariepinus Polymorphism, Antiboitic
Highlights
Condition of use: The user may copy, distribute, transmit and adapt the work, but must recognize the authors and the Nigerian Journal of Biotechnology
According to (Olaoye et al, 2013), millions of bacteria, many of which are potential spoilers are present in the guts, in the gills and in the surface slime covering the skin of fish, though the skin is the first layer of defense, and is involved in both physical and immune defenses against pathogenic bacteria, viruses and parasites
The main objective of this study is to characterize and identify the bacteria associated with C. gariepinus from Ajilete location on Yewa River using the RAPDPCR method and to evaluate the bacteria susceptibility to antibiotics
Summary
Condition of use: The user may copy, distribute, transmit and adapt the work, but must recognize the authors and the Nigerian Journal of Biotechnology. The least inhibition zone was recorded in Cotrimoxazole on the second sample with (8 mm), 70.5 % bacteria strains were susceptible to Gentamicin while two samples displayed 100 % resistance to all the antibiotics. Fish is a vital food source for people; it is the most important single source of high-quality protein, providing approximately 16 % of the animal protein consumed by the world's population, according to (FAO, 1997). This research was carried out to develop a baseline data on bacteria associated with C. gariepinus from Ajilete location on Yewa River as a result of the very few research works that have been carried out on this river. The main objective of this study is to characterize and identify the bacteria associated with C. gariepinus from Ajilete location on Yewa River using the RAPDPCR method and to evaluate the bacteria susceptibility to antibiotics
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