Genetic diversity of the pathogenic fungus Sarocladium oryzae causing sheath rot on rice using rep-PCR

Abstract

Rice becomes the main commodity cultivated in Asia. Sheath rot disease becomes one of the challenges in increasing rice production. Sarocladium oryzae is known to be one of the major pathogens in causing sheath rot disease. This pathogen has been reported in several rice production countries, such as Brazil, India, Taiwan, Philippines, Thailand, including Indonesia, and causing yield loss of up to 85%. The study of genetic diversity is important to investigate the rapid shift in genetic structures. A total of six S. oryzae strains showed diverse in molecular using repetitive-PCR (rep-PCR). The isolate SO8 and SO13 isolate SO2 and SO11 and isolate SO3, and SO5 has the relatively same the band patterns in BOX, ERIC, and REP sets of primers. The results of the UPGMA analysis show that S. oryzae in the various primers can be divided into three different group consistency. This result indicates that the rep-PCR method can genetically distinguish S. oryzae. The use of the three main sets, BOX, ERIC, and REP, suggested differentiating the other fungal species.