Genetic diversity of Scrophularia ningpoensis based on SCoT analysis

Abstract

To explore the genetic diversity and relationship of different Scrophularia ningpoensis cultivars. Forty-eight germplasmic resources of S. ningpoensis cultivars were analyzed by Start Codon Targeted Polymorphism(SCOT) molecular markers. Genetic distance was calculated by TREECONW software and the systematic diagram of genetic relationship was clustered by UPGMA method. A total of 279 bands were detected using 48 primers, among which 214 were polymorphic bands. The average percentage of polymorphic bands was 76.7%. Genetic distance was changed from 0.1507 to 0.4933. Clustering results showed that the genetic relationship of S. ningpoensis cultivars was more complex. There was significant correlation between some germplasm and its geographic origin while geographical distribution of some germplasm was not very obvious, but it was also showed that some of the S. ningpoensis from the same region were in the same group which presented the law of geographical distribution in the tested materials. Significant polymorphism and genetic diversity can be observed among S. ningpoensis germplasm resources which provided a wealth of genetic basis for cultivating fine varieties.